Illumina adapter sequences pdf

Product documentationavailable for download in pdf from the illumina website. Because the distance between each paired read is known. Dual indexing on the following instruments requires the reverse complement of the index 2 15 adapter sequence. An introduction to illumina nextgeneration sequencing technology for microbiologists. These sequences are provided for the sole purpose of understanding and publishing the results of your sequencing. Indexing and barcoding for illumina nextgen sequencing elk 10. We can order those for you through the core lab, but there. At this time, illumina truseq adapters and primers are only available in the truseq kits from illumina. Without everything exactly right you are liable to get no data from your. Index1i7 adapters i7indexname i7basesforsamplesheet a701 atcacgac a702 acagtggt a703 cagatcca a704 acaaacgg a705 acccagca a706 aacccctc a707 cccaacct a708 caccacac a709 gaaaccca a710 tgtgacca a711 agggtcaa a712 aggagtgg index2i5 adapters i5indexname i5basesforsamplesheet novaseq,miseq,hiseq20002500 i5basesforsamplesheet iseq,miniseq,nextseq. What is the sequence of illumina universal adapters. This information is provided for use with illumina instruments only. These sequences are provided for the sole purpose of understanding and publishing the results of your sequencing experiments. Overview of indexed sequencing on the nextseq, miseq, and.

Pdf illumina sequencing library preparation for highly. Sample preparation for wholegenome sequencing at a glance cebased sanger sequencing nextgeneration sequencing. Indexes and full length adapter sequences are added by amplification with 5 tailed primers steps 2226. This document lists the adapter sequences for illumina library prep kits. Understanding illumina truseq adapters tufts genomics. Illumina sequencing library preparation for highly multiplexed target capture and sequencing article pdf available in cold spring harbor protocols 20106. Dualindexed libraries add index 1 i7 and index 2 i5 sequences to. However, it would be really helpful if anyone could provide the sequence of illumina universal adapters. The key to sequencing random fragments of dna is by the addition of short nucleotide. Our libraries make use of standard illumina sequencing primers and index.

This document provides the nucleotide sequences that comprise illumina oligonucleotides used in illumina sequencing technologies. If you plan to create your own adapter sequences, indexes, and primers it is essential to know what you are doing. The sequences are grouped into sections for trusight kits, nextera kits, and truseq kits, with an appendix that lists truseq controls and information for legacy illumina kits. This document provides the nucleotide sequences that comprise illumina. During illumina library preparation, sample dna is fragmented, endrepaired and atailed. Oligonucleotide oligo sequences of illumina adapters used in ampliseq, nextera, truseq, and trusight library prep kits. Sequencing control software on illumina sequencing platforms processes these tags in an. Illumina sequencing library preparation for highly. Illumina adapter sequences 000002694 dna technologies. An introduction to illumina nextgeneration sequencing. Pairedend sequencing pairedend pe sequencing, where both ends of a dna fragment are sequenced figure 4 allows long range positioning of the dna fragment.